Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bifidobacterium longum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.165 | - |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | pH 5.0, 37°C | Bifidobacterium longum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Bifidobacterium longum | 9986 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
predicted from amino acid sequence | Bifidobacterium longum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium breve | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium breve JCM 1192 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum ATCC 29521 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum JCM 7054 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium longum JCM 1217 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | Bifidobacterium bifidum JCM 7004 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium breve | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium breve JCM 1192 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum ATCC 29521 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum JCM 7054 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium longum JCM 1217 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | Bifidobacterium bifidum JCM 7004 | - |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
ATCC29521 | - |
Bifidobacterium bifidum | - |
JCM 1254 | - |
Bifidobacterium bifidum | - |
JCM 7004 | - |
Bifidobacterium bifidum ATCC 29521 | - |
ATCC29521 | - |
Bifidobacterium bifidum JCM 7004 | - |
JCM 7004 | - |
Bifidobacterium breve | - |
JCM 1192 | - |
Bifidobacterium breve JCM 1192 | - |
JCM 1192 | - |
Bifidobacterium longum | - |
JCM 7054 | - |
Bifidobacterium longum | Q3T552 | JCM 1217 | - |
Bifidobacterium longum JCM 1217 | Q3T552 | JCM 1217 | - |
Bifidobacterium longum JCM 7054 | - |
JCM 7054 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bifidobacterium longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium breve | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium breve JCM 1192 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum ATCC 29521 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum JCM 7054 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium longum JCM 1217 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O | - |
Bifidobacterium bifidum JCM 7004 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium breve | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium breve JCM 1192 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum ATCC 29521 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum JCM 7054 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium longum JCM 1217 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O | - |
Bifidobacterium bifidum JCM 7004 | 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein] | - |
? | |
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum | p-nitrophenol + Gal-beta-1,3-GalNAc | - |
? | |
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum JCM 7054 | p-nitrophenol + Gal-beta-1,3-GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium breve | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium breve JCM 1192 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum ATCC 29521 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium longum JCM 1217 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O | pH 5.0, 37°C | Bifidobacterium bifidum JCM 7004 | p-nitrophenol + Galbeta(1,3)GalNAc | - |
? | |
additional information | Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | Bifidobacterium longum | ? | - |
? | |
additional information | Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | Bifidobacterium longum JCM 1217 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 210000, SDS-PAGE | Bifidobacterium longum |
Synonyms | Comment | Organism |
---|---|---|
endo-alpha-GalNAc-ase | - |
Bifidobacterium bifidum |
endo-alpha-GalNAc-ase | - |
Bifidobacterium breve |
endo-alpha-GalNAc-ase | - |
Bifidobacterium longum |
endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium bifidum |
endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium breve |
endo-alpha-N-acetylgalactosaminidase | - |
Bifidobacterium longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bifidobacterium longum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme is stable up to 37°C and retains 73% activity after incubation at 45°C for 30 min in 50 mM acetate buffer (pH 5.0) | Bifidobacterium longum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol | pH 5.0, 37°C | Bifidobacterium longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
at 37°C in 10 mM acetate buffer | Bifidobacterium longum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | 68% activity at pH 4.0, 73% activity at pH 6.0 | Bifidobacterium longum |